xbp1 stalling sequence Search Results


xbp1 stalling sequence  (Addgene inc)
91
Addgene inc xbp1 stalling sequence
a-e, Reporter constructs shown on the left were transfected into HEK293T cells. The EGFP/mCherry ratio was quantified in individual cells using flow cytometry with distributions shown on the right on a log-10 scale. The number in each plot is the median fold-decrease of the EGFP/mCherry ratio. Note that AMD1 sequence causes less decrease in EGFP compared to both <t>XBP1</t> and poly(A) sequences, and even this weak effect is independent of the putative pausing sequence in AMD1.
Xbp1 Stalling Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xbp1+stalling+sequence/pmc10560126-273-0-6?v=Addgene+inc
Average 91 stars, based on 1 article reviews
xbp1 stalling sequence - by Bioz Stars, 2026-08
91/100 stars
  Buy from Supplier

Image Search Results


a-e, Reporter constructs shown on the left were transfected into HEK293T cells. The EGFP/mCherry ratio was quantified in individual cells using flow cytometry with distributions shown on the right on a log-10 scale. The number in each plot is the median fold-decrease of the EGFP/mCherry ratio. Note that AMD1 sequence causes less decrease in EGFP compared to both XBP1 and poly(A) sequences, and even this weak effect is independent of the putative pausing sequence in AMD1.

Journal: Nature

Article Title: Noncoding translation mitigation

doi: 10.1038/s41586-023-05946-4

Figure Lengend Snippet: a-e, Reporter constructs shown on the left were transfected into HEK293T cells. The EGFP/mCherry ratio was quantified in individual cells using flow cytometry with distributions shown on the right on a log-10 scale. The number in each plot is the median fold-decrease of the EGFP/mCherry ratio. Note that AMD1 sequence causes less decrease in EGFP compared to both XBP1 and poly(A) sequences, and even this weak effect is independent of the putative pausing sequence in AMD1.

Article Snippet: XBP1 stalling sequence was amplified from Addgene plasmid #159583 with Phusion PCR kit (New England Biolabs, # M0530S).

Techniques: Sequencing, Construct, Transfection, Flow Cytometry